Journal: Virology Journal

Article Title: The expression and significance of proto-oncogene c-fos in viral myocarditis

doi: 10.1186/1743-422X-7-285

Figure Lengend Snippet: The expression change of c-fos oncogene mRNA in VMC mice

Article Snippet: c-fos monoclonal antibody, isoproterenol, normal goat serum, rabbit anti-c-fos oncogene protein, Biotinylated goat anti rabbit IgG, Streptavidin Biotin-peroxidase Complex (SABC), antigen restoration solution, pepsin, c-fos oligonucleotide probe, Occlusive solution, and rabbit anti digoxin were purchased from Boster Biological Technology Ltd.(Wuhan, China), Sigma Chemical Co. (Sigma, St.Louis, MO) and Biocompare Co.(South San Francisco, CA).

Techniques: Expressing, Control

Journal: Virology Journal

Article Title: The expression and significance of proto-oncogene c-fos in viral myocarditis

doi: 10.1186/1743-422X-7-285

Figure Lengend Snippet: The expression change of c-Fos oncogene protein in VMC mice

Article Snippet: c-fos monoclonal antibody, isoproterenol, normal goat serum, rabbit anti-c-fos oncogene protein, Biotinylated goat anti rabbit IgG, Streptavidin Biotin-peroxidase Complex (SABC), antigen restoration solution, pepsin, c-fos oligonucleotide probe, Occlusive solution, and rabbit anti digoxin were purchased from Boster Biological Technology Ltd.(Wuhan, China), Sigma Chemical Co. (Sigma, St.Louis, MO) and Biocompare Co.(South San Francisco, CA).

Techniques: Expressing, Control

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 1 Serum adiponectin concentrations in rabbits. 1, Aorta non-injured rabbits on normal cholesterol diet; 2, aorta injured rabbits on high-cholesterol diet before adenovirus transfer; 3, after transfer Ad-bgal through intima; 4, after transfer Ad-APN through intima; 5, after transfer Ad-bgal through adventitia; 6, After transfer Ad-APN through adventitia. The symbol * indicates significant difference (P!0.01) vs serum adiponectin concentrations in aorta non-injured rabbits on normal cholesterol diet; † compared with rabbits before adenovirus transfer; and ‡ compared with rabbits after Ad-bgal transfer. The values are meansGS.E.M.

Article Snippet: Following blocking with 5% non-fat milk, the blots were incubated with anti-His tag mouse monoclonal antibody (1:1000 dilution, Applygen Technologies Inc., Beijing, China) and rabbit adiponectin polyclonal antibody (1:100 dilution, Boster, Wuhan, Hubei, China) at 4 8C overnight.

Techniques:

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 2 SDS-PAGE (A) and western blot analysis of the supernatant of cultured Pichia pastoris (B, using anti-His tag monoclonal antibody; C, using adiponectin polyclonal antibody). Line 1 is a blank pGAPZa Pichia vector (not containing adiponectin gene) sample control and lines 2–4 are samples from the supernatant of cultured Pichia pastoris containing the recombinant rabbit adiponectin gene. Line 5 contained the molecular weight markers (not visible in image of blot). Both anti-His antibody and adiponectin polyclonal antibody detected a single predominant protein at z42 kDa (containing 9.3 kDa a-factor signal peptide and 2.5 kDa C-terminal His-tag).

Article Snippet: Following blocking with 5% non-fat milk, the blots were incubated with anti-His tag mouse monoclonal antibody (1:1000 dilution, Applygen Technologies Inc., Beijing, China) and rabbit adiponectin polyclonal antibody (1:100 dilution, Boster, Wuhan, Hubei, China) at 4 8C overnight.

Techniques: SDS Page, Western Blot, Cell Culture, Plasmid Preparation, Control, Recombinant, Molecular Weight

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 5 Effect of Ad-APN treatment on adhesion molecules in atherosclerotic rabbits 14 days after recombinant adenovirus transfer. A, Immunohistochemical detection of adiponectin, VCAM-1 and ICAM-1 in atherosclerotic lesions. Representative images are shown. 1, Ad-bgal transfer through intima. 2, Ad-APN transfer through intima. 3, Ad-bgal transfer through adventitia. 4, Ad-APN transfer through adventitia. The magnification is 400. B, mRNA expression of VCAM-1 and ICAM-1 in abdominal aortic tissue in Ad-bgal or Ad-APN-treated rabbits. The values are meansGS.E.M.

Article Snippet: Following blocking with 5% non-fat milk, the blots were incubated with anti-His tag mouse monoclonal antibody (1:1000 dilution, Applygen Technologies Inc., Beijing, China) and rabbit adiponectin polyclonal antibody (1:100 dilution, Boster, Wuhan, Hubei, China) at 4 8C overnight.

Techniques: Recombinant, Immunohistochemical staining, Expressing

Journal: eLife

Article Title: A zebrafish and mouse model for selective pruritus via direct activation of TRPA1

doi: 10.7554/elife.32036

Figure Lengend Snippet: Figure 2. Trpa1b, but not Tlr7, is required for both neuronal and behavioral responses to IMQ. (A, B) In situ hybridization in 3dpf WT larvae probing for tlr7 and trpa1b mRNA respectively. (C, D) Larval locomotor assay of 5dpf tlr7+/+ and tlr7-/- (C) or trpa1b+/+ and trpa1b-/- (D) larvae. (E, F) Adult lip- rubbing behavioral assay of tlr7+/+/tlr7-/- (E) and trpa1b+/+/trpa1b-/-(F) fish. (C), (D), (E), (F), 100 mM IMQ used. ***p<0.001, **p<0.01, Student’s t-test. Bars represent mean ± s.e.m. (G, H) Representative calcium imaging traces of 3dpf trpa1b+/+ (G) and trpa1b-/- (H) larvae in a transgenic elavl3HuC: GCaMP5 background exposed to 100 mM IMQ, 50 mM AITC, and 1 mM 2-APB. B = blood, TG = trigeminal ganglion. DOI: https://doi.org/10.7554/eLife.32036.008 The following figure supplement is available for figure 2:

Article Snippet: Primary antibodies against TLR7 (rabbit anti-TLR7, Boster, Pleasanton, CA, 1:250) and GFP (chick anti-GFP, 1:1000, Invitrogen) were made in PBST with 10% goat serum and applied to the coverslips, which were Esancy et al. eLife 2018;7:e32036.

Techniques: In Situ Hybridization, Behavioral Assay, Imaging, Transgenic Assay

Journal: eLife

Article Title: A zebrafish and mouse model for selective pruritus via direct activation of TRPA1

doi: 10.7554/elife.32036

Figure Lengend Snippet: Figure 3. Imiquimod directly activates TRPA1. (A–C) Calcium imaging of HEK cells transfected with zebrafish (A), mouse (B), and human (C) Trpa1 or Trpa1 + Tlr7. (D, E, F) Calcium imaging dose response curves of HEK cells transfected with zebrafish (D), mouse (E), and human (F) Trpa1, exposed to IMQ or AITC. (A–F) Numbers represent total cell counts per condition. (G) Patch clamp of HEK cell transfected with zebrafish trpa1b exposed to 100 mM IMQ. (H) Patch clamp dose response curve for HEK cells transfected with zebrafish trpa1b, mouse Trpa1, or mouse Trpa1 + mouse Tlr7. (I) Current density values of HEK cells exposed transfected with zebrafish trpa1b or mouse Trpa1 and exposed to 100 mM AITC or 100 mM IMQ. (G–I) n = 5 cells per condition. **p<0.01, Student’s t-test. Bars represent mean ± s.e.m. DOI: https://doi.org/10.7554/eLife.32036.010 The following figure supplements are available for figure 3:

Article Snippet: Primary antibodies against TLR7 (rabbit anti-TLR7, Boster, Pleasanton, CA, 1:250) and GFP (chick anti-GFP, 1:1000, Invitrogen) were made in PBST with 10% goat serum and applied to the coverslips, which were Esancy et al. eLife 2018;7:e32036.

Techniques: Imaging, Transfection, Patch Clamp